introduction
Inflammation is a physiological response that protects the body against pathogens, stresses and tissue damage. Despite this, it can be a factor of many chronic diseases including diabetes, atherosclerosis, arthritis, and ulcerative colitis when chronic inflammation occurs [1].
Lipoxygenase (LO) is a group of oxidative enzymes that participates in metabolic process of archidonic acid into chemical transmitter in inflammatory reaction and is involved in the regulation for the responses of inflammation by generating pro-inflammatory mediators [2].
Nonsteroidal anti-inflammatory drugs (NSAIDs) are one of the most commonly used pain relievers in the world, but they have some adverse side effects. Thus, there is a current worldwide search towards finding alternative treatment from natural resources [3].
The Vigna radiata L. commonly known as mung bean is a legume cultivated for its edible seeds and sprouts in the places of Asia. The V. radiata bean plant is an annual, erect or semi-erect, reaching a height of 0.15-1.25 m. The leaves are alternate, trifoliolate with elliptical to ovate leaflets, measuring 5-18 cm long x 3-15 cm broad [4].
Bioactive plant compounds are produced as secondary metabolites that are not vital for the functioning of the plant, but play an important role in the composition, defense, attraction, and signaling [5].
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Due to the limited studies on the presence of bioactive compounds in V. radiata leaf, results could benefit the research community most as this study provides preliminary analysis of V. radiata leaf.
This study is limited in screening the phytochemical compounds present in V. radiata leaf only. Results from the phytochemical analysis will be used in determining the anti-inflammatory property of V. radiata leaf through in-vitro lipoxygenase inhibition.